Vol. 3, No 1, 2005 pp. 11 - 22
UDC 611.36:159.9.072
Scientific Paper
EFFECTS OF ACUTE ETHIONINE-INDUCED
HEPATIC
ATP DEFICIENCY AT REST AND DURING EXERCISE IN FEMALE RATS
Abbass Ghanbari-Niaki1, Francois
Desy2, Jean-Marc Lavoie2
1Department of Physical Education,
Faculty of Hymanities,
Tarbiat Modares University, Teheran, Iran, E-mail: ghanbary@modares.ac.ir
2Departement de kinesiologie,
Universite
de Montreal (Quebec), Canada H3C 3J7,
E-mail: jean-marc.lavoie@umontreal.ca
Abstract. The effects of an injection (ip) of ethionine (1.0
mg/g)
with and without methionine (1.0 mg/g) were studied at rest and
following
a 30-min bout of exercise (26 m/min, 0% grade) in female Sprague-Dawley
rats. Ethionine, compared to a NaCl injection, resulted in a
significant
(P < 0.05) decrease in resting levels of liver ATP, liver glycogen,
and plasma glucose and in a significant increase in liver Pi/ATP ratio
and plasma FFA, glycerol, glucagon, and norepinephrine concentrations.
All of these responses were restored by the addition of methionine.
None
of these responses were amplified by the combination of ethionine and
exercise
stimuli. These results indicate a predominance of ethionine over the
exercise
stimulus and suggest that intra hepatic events related to a decreased
ATP
level in the liver exert a powerful regulatory influence on the
metabolism.
Key words: liver ATP, glucagon, liver glycogen, hepatic
receptors, liver inorganic phosphates.
POSLEDICE AKUTNOG ETIONINOM IZAZVANOG
HEPATINOG STANJA
KOD ŽENKI PACOVA PRILIKOM MIROVANJA I VEŽBANJA
Ova studija se bavi ispitivanjem efekata ubrizgavanja (ip) etionina
(1.0
mg/g) sa i bez metionina (1.0 mg/g) ženkama Sprague-Dawley pacova u
stanju
mirovanja i nakon 30-minutnog vezbanja (26m/min, 0% nivo). Etionin, u
poredjenju
sa ubrizgavanjem NaCl ima za rezultat značajno smanjenje jetrinog ATP
(P<0.05),
jetrinog glikogena glukoze plazme u stanju mirovanja, i značajno
povećanje
odnosa jetrinog Pi/ATP I plazme FFA, glikerola, glukagona, i
koncentracija
norepinefrina. Sve ove analize su ponovo vraćene na stari nivo
dodavanjem
metionina. Nijedna od ovih analiza nije bila uvećana kada je etionin
bio
kombinovan sa varijablom- vežbanje. Ovi rezultati ukazuju na
predominantnost
etionina nad stimulativnim vežbanjem i sugerišu da intra-hepatična
stanja
povezana sa umanjenjem nivoa ATP-a u jetri imaju snažan regulacioni
uticaj
na metabolizam.
Ključne reči: jetreni ATP, glukagon, jetreni glukogen, hepatični
receptori, jetreni neorganski fosfati.
