Vol.1, No 5, 2000 pp. 87 - 93
UDC 582.824
THE EXTRACTION OF APIGENIN AND LUTEOLIN
FROM THE  SAGE SALVIA OFFICINALIS L. FROM JORDAN
Siniša Đorđević1, Milorad Cakić1, Salameh Amr2
1Faculty of Technology, Bulevar oslobodjenja 124, Leskovac, Yugoslavia
2Royal Science Institute, Amman- Alqwesme, Box 38365, Jordan
Abstract. In the Kingdom of Jordan first class sage (maramia) (Salvia officinalis L.) grows both in wilderness and cultivated on plantations, and it is the most important plant species in the country.
Our aim is to investigate the chemical composition of the sage originating from Jordan, and in this paper the original procedure is described for the extraction of apigenin and luteolin.
The preparative chromatography on paper was used for the isolation of these compounds from  ethanol extract (1:10) of the plant (flower and top leaves) in the n-butanol-water-acetic acid system 12:2:1 v/v/v.
Considering the Rf values of IR and UV spectra (with and without adding the specific agents NaOMe, NaOAc, NaOAc+H3BO3, AlCl3 and AlCl3+Hcl) and compared with the literature data it was determined that the isolated compounds were apigenin and luteolin.
Key words: sage, extraction, apigenin, luteolin
IZOLOVANJE APIGENINA I LUTEOLINA IZ ŽALFIJE
SALVIA OFFICINALIS L. POREKLOM IZ JORDANA
U Kraljevini Jordan spontano raste, ali se i plantažno gaji žalfija (maramija) (Salvia officinalis L.) izvanrednog kvaliteta i najvažnija je biljna vrsta u ovoj zemlji.
Naš cilj je istraživanje hemijskog sastava žalfije poreklom iz Jordana, a u ovom radu izložen je originalni postupak za izolovanje apigenina i luteolina.
Za izolovanje ovih jedinjenja primenjena je preparativna hromatografija na papiru etanolnog ekstrakta (1:10) biljke (cvet + gornji lisni deo) u sistemu n-butanol-voda-sirćetna kiselina 12:2:1 v/v/v.
Na osnovu Rf vrednosti, IR i UV spektra (sa i bez dodataka specifičnih reagensa NaOMe, NaOAc, NaOAc+H3BO3, AlCl3 i AlCl3+HCl) kao i poredjenjem sa literaturnim podacima utvrdjeno je da su izolovana jedinjenja apigenin i luteolin.
Ključne reči: žalfija, ekstrakcija, apigenin, luteolin