Vol.1, No 5, 1998 pp. 129 - 134
UDC: 535.243+661.727.2+631.867
A METHOD FOR SPECTROPHOTOMETRIC DETERMINATION
OF ACETALDEHYDE
WITH THIOSEMICARBAZIDE IN BLOOD
Vidosava Djurdjić, Srdjan Stojanović
Faculty of Chemistry, University of Belgrade
Studentski trg 16, 11000 Belgrade, Yugoslavia
Abstract. The thiosemicarbazide method for spectrophotometric determination
of acetaldehyde (AcH) in whole blood is presented. The concentration of
thiosemicarbazone formed in slightly acidic medium was determined at 260
nm. The influence of non-specific chromogens present in blood was eliminated
by simultaneous AcH distillation and thiosemicarbazone formation. The oxidation
of AcH in blood samples was prevented by chloral hydrate addition. The
presented method was found two folds more sensitive than a spectrophotometric
determination of AcH by semicarbazide. Due to its simplicity and sensitivity
as well as to the use of low cost equipment, this method should be a valuable
complement to expensive GLC or HPLC determinations of AcH.
Keywords: acetaldehyde (AcH), thiosemicarbazide, spectrophotometric
determination, thiosemicarbazone
SPEKTROFOTOMETRIJSKO ODREĐIVANJE ACETALDEHIDA
U KRVI SA TIOSEMIKARBAZIDOM
U radu je opisana metoda za spektrofotometrijsko određivanje acetaldehida
(AcH) u krvi. Kao reagens upotrebljen je tiosemikarbazid a apsorpcija nastalih
u slabo kiseloj sredini tiosemikarbazona određuje se na 260 nm. Uticaj
nespecifičnih hromogena prisutnih u krvi eliminiše se destilacijom AcH
sa istovremenim građenjem tiosemikarbazona. Oksidacija AcH u uzorcima krvi
sprečena je dodatkom hloral hidrata. Opisana metoda se pokazala osetljivom
u odnosu na spektrofotometrijsko određivanje AcH sa semikarbazidom. Zbog
svoje jednostavnosti, osetljivosti i ekonomičnosti ova metoda može predstavljati
korisnu zamenu za GLC i HPLC određivanja AcH.
Ključne reči: acetildehid (AcH), tiosemikarbazid, spektrofotometrijsko
određivanje, tiosemikarbazon
