Vol. 1, No 3, 1996 pp.
265 - 276
UDC
543 : 547.21
HOW TO INCREASE THE YIELD OF INDUCIBLE ENZYMES IN MICROORGANISMS
Ž. Petronijević
Faculty of Technology, Bulevar oslobođenja 124, 16000 Leskovac, Yugoslavia
Abstract.
The methods of increasing the yield of inducible enzymes (primarily) are listed in the paper. A number of methods are based on optimizing the cultivation conditions of the selecting producer, primarily by change of the growth medium composition; among the rest by application of inducers and by exclusion of components which cause catabolic repression or end-product repression. The other group of methods includes those based on obtaining and selecting mutants, both those constitutive and resistant to catabolic repression and those in which the increase of yield is accomplished by other mechanisms.
KAKO POVEĆATI PRINOS INDUCIBILNIH ENZIMA KOD MIKROORGANIZAMA
U radu su navedene metode za povećanje prinosa, pre svega, inducibilnih enzima. Jedan broj metoda je zasnovan na optimizovanju uslova kultivacije odabranog producenta, pre svega na promeni sastava hranljive podloge; između ostalog primenom induktora i isključenjem sastojaka koji izazivaju kataboličku represiju ili represiju konačnim proizvodom. Druga grupa metoda su one koje su bazirane na dobijanju i selekciji mutanata, kako onih konstitutivnih i otpornih na kataboličku represiju, tako i onih kod kojih do povećanja prinosa dolazi preko drugih mehanizama.
