Vol. 1, No 1, 1994 pp. 95 - 102
UDC
547.9:577.15
INFLUENCE OF THE LOW IONIC STRENGTHS ON THE IMMOBILIZATION OF DEXTRANSUCRASE
Ž. Petronijević1 , S. Jovanović2 , R. Nešić3
1Faculty of Technology, Durmitorska 19,16000 Leskovac, Yugoslavia
2Faculty of Technology and Metallurgy, P.O. Box 494,11001 Belgrade, Yugoslavia
3"ZDRAVLJE", Pharmaceutical and Chemical Works, Vlajkova 199,16000 Leskovac,Yugoslavia
Abstract. Influence of low ionic strengths (75 mM and 20 mM) on the immobilization process of dextransucrase enzyme on the hydrophobic carriers phenoxyacetyl and cinnamtyl cellulose has been tested. It has been established that the lowering of ionic strength leads to the decrease in quantities of bound proteins and to the low values for the immobilized activity. Immobilization process efficiency is also substantially decreased.
UTICAJ NISKIH JONSKIH JAČINA NA IMOBILIZACIJU DEKSTRANSAHARAZE
U radu je ispitivan uticaj niskih jonskih jačina (75 mM i 20 mM) na proces imobilizacije enzima dekstransaharaze na hidrofobnim nosačima fenoksiacetil- i cinamoil-celulozi. Nađeno je da sniženje jonske jačine dovodi do smanjenja količine vezanih proteina i do niskih vrednosti za imobilisanu aktivnost. Efikasnost procesa imobilizacije se pri ovome takođe jako smanjuje.
