Vol.2, No 2, 2000 pp. 93 - 96
UDC 582.4+546.21]:613.15

THE OXYGEN RADICALS IN ASTRAGALUS ONOBRYCHIS AND OXYTROPIS PILOSA
D. Miladinović1, Lj. Miladinović2
1Department of Chemistry, Faculty of Medicine,  B. Tasković 81, 18000 Niš, Yugoslavia
2High school "B. Stanković", Voždova 27, 18000 Niš, Yugoslavia

Abstract. The quantities of O2?- and ?OH of Astragalus onobrychis Var Chlorocarpus (Gris.) Stoj et Stef. (Leguminosae) and Oxytropis pilosa (L.) DC. produced from three different growth stages during 1995 at the locations Korvin grad (near Doljevac) and Subotinac (near Aleksinac), Serbia, were examined. The highest quantity of O2?- was found in the root of Astragalus  in the seed forming stage (3053.49 nmol/g fresh material). The highest quantity of O2?- in Oxytropis was also found in its root but in the blooming stage (2894.56 nmol/g fresh material). The root of Astragalus contained the highest quantity of ?OH (30.20 nmol/g fresh material) in the blooming stage. The leaves of Oxytropis in the same stage of growth contained 15.90 nmol/g fresh material.
Key words: Astragalus onobrychis Var. Chlorocarpus (Gris.) Stoj. et Stef. (Leguminosae), Oxytropis pilosa (L.) DC., oxygen radicals, leguminosae.

KISEONIČNI RADIKALI
U ASTRAGALUS ONOBRYCHIS I OXYTROPIS PILOSA
Ispitivana je količina O2?- i ?OH u korenu, stablu i listu vrsta Astragalus onobrychis Var. Chlorocarpus (Gris.) Stoj. et Stef. (Leguminosae) i Oxytropis pilosa (L.) DC. sa staništa Korvin grad (u blizini Doljevca) i Subotinac (u blizini Aleksinca) tokom vegetacionog perioda u 1995. godini. Najveća količina O2?- je ustanovljena u korenu Astragalus-a (3053,49 nmol/g, svež uzorak) u fazi formiranja semena. U korenu Oxytropis-a je takođe utvrđena najveća količina superoksid radikala (2894,56 nmol/g, svež uzorak) ali u fazi cvetanja. Koren Astragalus-a u fazi cvetanja sadrži najviše ?OH (30,20 nmol/g, svež uzorak). List  Oxytropis-a u istoj fazi vegetacije sadrži 15,90 nmol/g, svež uzorak, ?OH..