Vol. 11, No 1, 2013 pp. 67 - 73
DOI 10.2298/FUPCT1301067R
UDC 547.583.5 : 615.244
INFLUENCE OF METHYL AND ISOPROPYL N-METHYL ANTRANILATES ON CARBON TETRACHLORIDE-INDUCED CHANGES IN RAT LIVER MORPHOLOGY AND FUNCTION
Niko S. Radulović1, Pavle J. Randjelović2, Nikola M. Stojanović3, Ivan R. Ilić4, Ana B. Miltojević1
1Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000 Niš, Serbia
2Department of Physiology, Faculty of Medicine, University of Niš,
Zorana Đinđića 81, 18000 Niš, Serbia
3Faculty of Medicine, University of Niš, Zorana Đinđića 81, 18000 Niš, Serbia
4Institute of Pathology, Faculty of Medicine, University of Niš,
Zorana Đinđića 81, 18000 Niš, Serbia
E-mail: nikoradulovic@yahoo.com
Abstract. The aim of the present study was to examine potential protective effects of methyl N-methylanthranilate (M) and isopropyl N-methylanthranilate (I) in a rat model of acute intoxication with carbon tetrachloride (CCl4) by tracking the changes in liver morphology and function. Serum transaminase and bilirubin were significantly elevated in animals treated with CCl4 alone. A pretreatment with M and I prior to the administration of CCl4 significantly prevented the increase of serum levels of liver damage markers. Histopathological evaluation of the livers of the test animals also revealed that M and I reduced the incidence of liver lesions. Our experiments showed that both M and I possess protective effect in CCl4-induced liver damage in rats. The results are of interest due to the presence of natural or synthetic M in the human diet.
Key words: carbon tetrachloride, methyl and isopropyl N-methyl anthranilates, liver, transaminases
UTICAJ METIL- I IZOPROPIL-N-METILANTRANILATA NA UGLJEN-TETRAHLORIDOM IZAZVANE PROMENE U FUNKCIJI I MORFOLOGIJI JETRE PACOVA
U ovom radu ispitivano je potencijalno protektivno dejstvo metil N-metilantranilata (M) i izopropil N-metilantranilata (I) u modelu akutne intoksikacije pacova sa ugljen-tetrahloridom (CCl4) praćenjem promena u morfologiji i funkciji jetre. Kod životinja tretiranih sa samo CCl4 značajno su bile povećane vrednosti transaminaza i bilirubina u serumu. Pretretman sa M i I značajno je umanjio porast serumskih markera oštećenja jetre. Patohistološka analiza tkiva jetre pokazala je da M i I smanjuju stepen oštećenja jetre. Ovaj eksperiment je pokazao da i M i I imaju protektivno dejstvo kod oštećenja jetre izazvanog CCl4. Ovi rezultati su značajni zbog prisustva prirodnog ili sintetskog M u ljudskoj ishrani.
Ključne reči: ugljen-tetrahlorid, metil- i izopropil-N-metilantranilati, jetra, transaminaze
