Vol. 11, No 1, 2013 pp. 27 - 43
DOI 10.2298/FUPCT1301027A
UDC 681.586.74 : 577.212
ELECTROCHEMICAL BIOSENSORS AS A TOOL FOR THE INVESTIGATION OF DNA STRUCTURE, DAMAGE AND INTERACTION WITH OTHER MOLECULES
Mara M. Aleksić1, Vera Kapetanović2
1University of Belgrade, Faculty of Pharmacy, Department of Physical Chemistry and Instrumental Methods, Vojvode Stepe 450, 11221 Belgrade, Serbia
2University of Belgrade, Faculty of Pharmacy, Department of Analytical Chemistry, Vojvode Stepe 450, 11221 Belgrade, Serbia
E-mail: mara@pharmacy.bg.ac.rs
Abstract.In recent years, a great progress was achieved in the development of electrochemical sensors for DNA sequences, hybridization and damage. Nowadays, electrochemical methods are able to detect DNA at nanomolar concentration. In addition, these methods are suitable for studding both covalent and non-covalent binding interactions between DNA and different small molecules, e.g. drugs or potentially mutagenic agents. This suggests that electrochemical biosensors might become important tools in medical research. The aim of this review is to draw attention to the applicability of different electrochemical techniques for studying interactions between DNA with other molecules, and in the design of new sensitive and selective biosensors.
Key words: Electrochemical biosensor, DNA, binding interaction, damage
PRIMENA ELEKTROHEMIJSKIH BIOSENZORA ZA ISPITIVANJE STRUKTURE, OŠTEĆENJA I INTERAKCIJA DNK SA DRUGIM MOLEKULIMA
Poslednjih godina je postignut veliki napredak u razvoju elektrohemijskih biosenzora za ispitivanja sekvenci, hibridizacije i oštećenja na molekulu DNK. Primenom elektrohemijskih metoda se u današnje vreme mogu detektovati i određivati nanomolarne koncentracije DNK. Pored toga, ove metode su pogodne za ispitivanje kako kovalentnih, tako i nekovalentnih interakcija između DNK i različitih malih molekula, kakvi su lekovi i drugi potencijalno mutageni agensi. Ovo sugeriše da bi elektrohemijski biosenzori mogli biti od značaja i u oblast medicinskih istraživanja. Cilj ovoga preglednog rada je da skrene pažnju na primenljivost različitih elektrohemijskih metoda za proučavanje interakcija između DNK i drugih molekula, kao i u izradu novih osetljivih i selektivnih biosenzora.
Ključne reči: elektrohemijski biosenzor, DNK, interakcije, oštećenja