Vol.6, No 1, 2008 pp. 105 - 117
DOI 10.2298/FUPCT0801105T
UDC 539.2
DEVELOPMENT OF TIME-RESOLVED LASER-INDUCED
FLUORESCENCE SPECTROSCOPIC TECHNIQUE
FOR THE ANALYSIS OF BIOMOLECULES
M. Terziċ1,2, B. P. Marinkoviċ1,3,
D. Ševiċ1,3, J. Jureta1,
A. R. Milosavljeviċ1
1Institute of Physics, Pregrevica 118,
11080 Belgrade, Serbia
2Faculty of Sciences, University of
Novi Sad, Trg Dositeja Obradoviċa 4, 2100 Novi Sad, Serbia
3The Advanced School of Electrical
Engineering and Computing, Vojvode Stepe 283, 11000 Belgrade, Serbia
Abstract. Our developments of the time–resolved laser–induced
fluorescence (TR–LIF) detection system for biomolecules are presented.
This system is based on the tunable (320 nm to 475 nm) Nd:YAG laser pulses
used to excite various biomolecules. The detection part is the Streak System
for Fluorescence Lifetime Spectroscopy (Hamamatsu, Japan). The system consists
of a C4334–01 streakscope, as a detector, DG 535 digital pulse/delay
generator, C5094–S Spectrograph and HPD–TA System, as a temporal analyzer.
The TR–LIF spectrometer is designed primarily to study the temperature
and pressure effects on fluorescence behavior of biomolecules upon excitation
with a single nanosecond pulse. The design of this system has capability
to combine laser–induced breakdown (LIB) with fluorescence, as well to
study optodynamic behavior of fluorescence biomolecules.
Key words: Fluorescence, Laser–induced spectroscopy, biomolecules,
optodynamic
RAZVOJ VEMENSKI RAZLOŽENE LASERSKI INDUKOVANE
FLUORESCENTNE SPEKTROSKOPSKE TEHNIKE
ZA ANALIZU BIOMOLEKULA
Prikazan je razvoj vremenski-razloženog laserski indukovanog fluorescentnog
(TR–LIF) detekcionog sistema za biomolekule. Sistem se zasniva na podesivim
(320 nm do 475 nm) Nd:YAG laserskim impulsima za pobudu različitih biomolekula.
Detekcioni deo čini „streak" sistem za fluorescentnu spektroskopiju
vremena života (Hamamatsu, Japan). On se sastoji od C4334–01 streakscope,
kao detektora, DG 535 digitalog impulsog/delay generatora, C5094–S spektrografa
i HPD–TA sistema za vremensku analizu. TR-LIF spektrometar je prevashodno
dizajniran za proučavanje temperaturnih efekata i efekata pritiska na
fluorescentno ponašanje biomolekula prilikom njihove ekscitacije pojedinačnim
nanosekundnim impulsom. Sistem je projektovan tako da omogućuje i kombinaciju
laserski indukovanog proboja (LIB) sa fluoroscencijom, kao i za proučavanje
optodinamičkog ponašanja fluorescentnih molekula.
