Vol.5, No 1, 1998 pp. 54 - 57
UDC: 578.08
THE NUMERICAL DENSITY OF IMMUNOGLOBULIN PRODUCING CELLS
IN DISEASED PALATINE TONSILS
Verica Avramović1, Predrag Vlahović2, Milan Stanković3
Running title: Immunoglobulin producing cells in palatine tonsil
1Institute of Histology and Embryology, Faculty of Medicine, Niš,
2Institute of Nephrology and Hemodialysis, Clinical Centre, Niš, 3Clinic of Otorhinolaryngology, Clinical Centre, Niš, Yugoslavia
Summary. The number of immunoglobulin (Ig)-producing cells per mm3 of tonsillar tissue, i.e., numerical density and their distribution in tonsils from  patients with idiophatic tonsillar hyperplasia (ITH), chronic hyperplastic tonsillitis (CHT) and recurrent tonsillitis (RT) were analysed. The numerical density was determined on serial sections of paraffin embedded tonsillar tissue stained by peroxidase-antiperoxidase (PAP) method. The number of IgA- and IgG-producing cells was significantly decreased in ITH, increased in RT, and unchanged in CHT in comparison with control group. In ITH and CHT numerical density of IgM -producing cells was increased whereas no difference in RT was registered. The obtained results could provide information relevant to the immunological competence of diseased tonsils.
Key words: Human palatine tonsil, stereology, immunoglobulin producing cells, tonsillitis
NUMERIČKA GUSTINA IMUNOGLOBULIN PRODUKUJUĆIH ĆELIJA
U OBOLELIM PALATINALNIM TONZILAMA

Analizirani su broj imunoglobulin (Ig)-produkujućih ćelija po mm3 tonzilarnog tkiva - numerička gustina i njihova distribucija u tonzilama pacijenata sa idiopatskom tonzilarnom hiperplazijom (ITH), hroničnim hiperplastičnim tonzilitisom (CHT) i rekurentnim tonzilitisom (RT). Numerička gustina je određivana na serijskim isečcima tkiva tonzile koje je ukalupljeno u parafin i bojeno peroksidaza-antiperoksidaza (PAP) metodom. Broj IgA- i IgG-produkujućih ćelija je bio značajno snižen u ITH, povećan u RT, i nepromenjen u CHT u poređenju sa  kontrolnom grupom. U ITH i CHT numerička gustina IgM-produkujućih ćelija je bila povećana, dok nije uočena razlika u RT. Dobijeni rezultati mogu predstavljati informaciju koja je relevantna za imunološku kompetenciju obolelih tonzila.
Ključne reči: Tonzila palatina čoveka, stereologija, immunoglobulin produkujuće ćelije, tonzilitis