Vol.5, No 1, 1998 pp. 50 - 53
UDC: 618.81
NEW METHOD FOR DYEING OF ELASTIC TISSUES:
MODIFICATION OF VERHOEFF'S METHOD
Slavoljub Petrović, Mirjana Abramović
Institute of Histology and Embryology and Institute of Medical Chemistry,
Faculty of Medicine, Niš Yugoslavia
Summary. The classical methods for dyeing of elastica in the light
microscopy understand the differentiation of the stain from tissue without
accomplishing the selective removing of the stain from the elastic tissue
surrounding. By our modification or Verhoeff's method in which the concentration
of iron (III) - chloride combined with 1% water solution of iodine in potassium-iodide
(1:1) is lessened, the successful dyeing of elastica is accomplished. The
differentiation of the sedimentous excess of Fe-hematein by iron (III)
- chloride usually used is successfully replaced by 0,5 % water solution
of Bismark Brown Y diazo dye in acetate buffer (2:1). The suggested diazo
dye in acid solution shows a great affinity to environment and amli to
elastic tissue differentiating indirectly the exessive Fe-hematein from
the surrounding of elastic tissues. The time of contrasting is not strictly
determined because there is no danger of rinsing the stain out of the elastic
tissues and lamellas. The elastica dyed by our modified method shows a
greater number of tissues and lamellas, it is more noticeable and its appearance
reflects their real width, length and net.
Key words: Elastic tissue, hystochemical identification
NOVA METODA ZA BOJENJE ELASTIČNIH VLAKANA
MODIFIKOVANOM VERHOEFF-OVOM METODOM
Klasične metode za bojenje elastike, u svetlosnoj mikroskopiji, podrazumevaju
diferencijaciju boje sa tkiva pri čemu se ne može postići selektivno odstranjivanje
boje isključivo sa okoline elastičnih vlakana. Našom modifikacijom Verhoeff-ovog
postupka u kome je smanjena koncentracija gvožđe (III)-hlorida u kombinaciji
sa 1% vodenim rastvorom joda u kalijum-jodidu (1:1) postignuto je uspešno
bojenje elastike. Diferencijacija viška istaloženog Fe-hemateina pomoću
gvožđa (III)-hlorida koja se rutinski koristi uspešno je zamenjena 0.5%
vodenim rastvorom diazo boje Bismark Brown Y u acetatnom puferu (2:1).
Predložena diazo boja u kiselom rastvoru pokazuje veliki afinitet za okolinu
a amli za elastična vlakna pri čemu se na posredan način vrši diferencijacija
viška Fe hemateina sa okoline elastičnih vlakana. Vreme kontrastiranja
nije strogo određeno, jer ne postoji opasnost od ispiranja boje sa elastičnih
vlakana i lamela. Obojena elastika prema našem modifikovanom postupku ispoljava
veći broj vlakana i lamela, uočljivija je a njen izgled odslikava njihovu
stvarnu širinu, dužinu i umreženost.
Ključne reči: Elastična vlakna, histohemijska identifikacija
