Vol.15, No 1, 2008 pp. 20 - 23
UC 615.322
G2 STUDIES
OF ANTIMUTAGENIC POTENTIAL OF CHEMOPREVENTIVE AGENT CURCUMIN IN ALLIUM
CEPA ROOT MERISTEM CELLS
Loganathan Palani Kumar, Natarajan Paneerselvam
Research Centre and Post Graduate Studies in Botany, The Madura College,
Madurai-625 011, S. India
E-mail: npanneer1958@gmail.com
Summary. Turmeric has long been used as a spice and food colouring
agent in Asia. Curcumin is one of the active principles in Turmeric. In
this research the antimutagenic potential of curcumin in Allium cepa root
meristem cells was evaluated. The root tip cells were treated with sodium
azide at 200µg/ml and 300µg/ml for 1h. Curcumin was given at 5, 10 and
20 µg/ml for 18h (one cell cycle), prior to sodium azide treatment. The
tips were squashed after colchicine treatment and the cells were analyzed
for chromosome damage and mitotic index. Curcumin did not induce chromosomal
aberration in Allium cepa root tip cell. Sodium azide alone induced chromosomal
aberrations significantly with increasing concentrations. The chromosomal
aberrations were significantly reduced in root tips cells pretreated with
curcumin. The study reveals the antimutagenic potential of curcumin against
sodium azide induced chromosomal aberrations.
Key words: Curcumin, chromosomal
aberrations, sodium azide
G2 STUDIJE ANTIMUTAGENOG POTENCIJALA HEMOPREVENTIVNOG
AGENSA KURKUMINA U
ĆELIJAMA POKORIČNOG TKIVA KORENA VRSTE ALLIUM CEPA
Kratak sadržaj: Indijski šafran (turmerik) se dugo koristi kao
začin i agens za bojenje hrane u Aziji. Kurkumin je jedna od aktivnih
osnova šafrana. U istraživanjima je procenjen antimutageni potencijal
kurkumina u ćelijama pokoričnog tkiva korena vrste Allium cepa. Ćelije
vrha korena tretirane su natrijum azidom 200µg/ml i 300µg/ml sat vremena.
Dodato je 5, 10 i 20 µg/ml kurkumina tokom 18 časova (jedan ćelijski
ciklus), pre tretmana natrijum azidom. Vrhovi su iscedjeni posle tretmana
kolcihinom i analizirano je oštećenje hromozoma i mitotični indeks ćelija.
Kurkumin nije izazvao aberaciju hromozoma ćelija vrha korena Allium cepa.
Samo sa povećanjem koncentracije natrijum azida povećavale su se aberacije
hromozoma. Hromozomne aberacije su bile značajno manje kod ćelija vrha
korena koje su prethodno bile tretirane kurkuminom. Studija je dokazala
antimutageni potencijal kurkumina kod aberacija hromozoma izazvanih natrijum
azidom.
Ključne reči: Kurkumin, aberacije
hromozoma, natrijum azid
