Vol.15, No 1, 2008 pp. 13 - 19
UC 631.466(540)
ISOLATION AND CHARACTERIZATION OF MARINE
ANTAGONISTIC ACTINOMYCETES FROM WEST COAST OF INDIA
Mohan Remya, Ramasamy Vijayakumar
Department of Microbiology, Dr.G.R. Damodaran College of Science (Autonomous)
Coimbatore – 641 014, Tamil Nadu, India
E-mail: rvijayakumar1979@yahoo.com
Summary. A total of 173 actinomycetes colonies were isolated from
near shore marine environment and mangrove ecosystem at 8 different locations
of Kerala, West Coast of India. Among them, 64 isolates were morphologically
distinct on the basis of spore mass colour, reverse side colour, aerial
and substrate mycelia formation and production of diffusible pigment. The
majority (47%; n=30) of these isolates were assigned to the genus Streptomyces.
Antimicrobial activities of isolates were also tested against various bacterial
and fungal pathogens. Of 64 isolates, 21 isolates had antimicrobial activity,
with 2 isolates showing broad spectrum of antimicrobial effect.
Key words: Soil actinomycetes
diversity, West Coast of India, antimicrobial activity, HPLC
IZOLACIJA I KARAKTERIZACIJA POMORSKIH
ANATAGONISTIČKIH AKTINOMICETA SA ZAPADNE OBALE INDIJE
Kratak sadržaj: Izolovano je ukupno 173 kolonija aktinomiceta sa
obližnje morske obale i ekosistema mangrova na 8 različitih lokcija Kerale,
na zapadnoj obali Indije. Medju njima, 64 izolata se morfološki razlikovalo
prema boji spora, boji naličja, formaciji vazdušnih i micelija podloge
i produkciji difuznog pigmenta. Većina (47%; n=30) izolata je pripisano
rodu Streptomyces. Antimikrobna aktivnost izolata je takodje testirana
na razne bakterijske i gljivične patogene. Od 64 izolata, 21 je pokazao
antimikrobnu aktivnost, od kojih je 2 izolata pokazalo širok spektar antimikrobnih
efekata.
Ključne reči: Raznolikost
aktinomiceta iz zemlje, zapadna obala Indije, antimikrobna aktivnost, HPLC
