Vol.13, No 2, 2006 pp. 109 - 113
UC 616.314:621.375.82
INVESTIGATION OF HeNe LASER THERAPY INFLUENCE
ON BCP/PLGA OSSEOINTEGRATION – EXPERIMENTAL
STUDY
Radmila Živković1, Ljiljana Kesić1,
Dragan Mihailović2, Nenad Ignjatović3,
Dragan Uskoković3
1Faculty of Medicine, Clinic of Stomatology, Niš
2Faculty of Medicine, Institute of Pathology, Niš
3Institute of Technical Sciences of SANU, Belgrade
E-mail: dr.rada@yahoo.com
Summary. The efficiency of HeNe laser application in treatment of
artificially caused bone defects in mandible of rats filled with biocomposite
BCP/PLGA (bicalcium phosphate/polylactide–co-glycolide) was analyzed. Animals
were divided in two separate groups. The first group (A) was the control
one. In the second group (B) the implantation site was submitted to HeNe
laser irradiation. Animals were sacrificed two, six, eight and twelve weeks
after laser therapy. Histological samples were decalcified and submerged
in paraffin blocks. Samples were colored with HE. Morphometrical analysis
consisted of measuring area fraction, area, integral optical density, mean
density and density variation. Results showed beneficial effect of laser
treatment in reparation of alveolar bone after two and six weeks. After
eight and twelve weeks no beneficial laser effect on biocomposite osseointegration
could be noticed.
Key words: Bicalcium phosphate/polylactide-co-glycolide,
HeNe laser, osseointegration
ISPITIVANJE UTICAJA HeNe LASEROTERAPIJE
NA BCP/PLGA OSEOINTEGRACIJU – EKSPERIMENTALNI
RAD
Kratak sadržaj: Ispitan je uticaj terapije HeNe laserom na arteficijalno
izazvane defekte mandibule pacova koji su ispunjeni sa biokompozitnim materijalom
BCP/PLGA (bifazni kalcijumfosfat/polilaktidkoglikolid). Životinje su podeljene
u dve grupe. Prva grupa (A) je bila kontrolna grupa. U drugoj grupi (B),
mesto implantacije biomaterijala je podvrgnuto dejstvu HeNe lasera. Životinje
su žrtvovane nakon dve, šest, osam i dvanaest nedelja nakon završene laseroterapije.
Histološki preparati su dekalcifikovani i ukalupljeni u parafinske blokove.
Preparati su bojeni HE. Morfometrijska analiza se satojala iz merenja arealne
frakcije, areje, integrisane optičke gustine, srednje gustine i varijacije
gustine koštanog tkiva. Rezultati su pokazali blagotvoran uticaj laseroterapije
u reparaciji koštanog tkiva nakon dve i šest nedelja. Nakon osam i dvanaest
nedelja nije uočen blagotvorni uticaj lasera na oseointegraciju biokompozita.
Ključne reči: Bifazni kalcijumfosfat/polilaktidkoglikolid,
HeNe laser, oseointegracija
