Vol.13, No 2, 2006 pp. 65 - 69
UC 618.19-006
QUANTIFICATION OF ARGYROPHILIC NUCLEOLAR
ORGANIZER REGIONS IN ESTROGEN RECEPTOR POSITIVE
AND ESTROGEN RECEPTOR NEGATIVE DUCTAL BREAST
CARCINOMAS
Žaklina Mijović1, Natalija Stefanović2,
Dragan Mihailović1, Miloš Kostov3
1Institute of Pathology, Faculty of Medicine, Niš, Serbia
2Institute of Anatomy, Faculty of Medicine, Niš, Serbia
3Department of Pathology, Military Hospital, Niš, Serbia
E-mail: zami@bankerinter.net
Summary. Argyrophilic nucleolar organizer regions (AgNORs) analysis
of 20 estrogen receptor positive (ER+) and 16 estrogen receptor negative
(ER-) invasive ductal breast carcinomas was performed. The quantification
of AgNORs was determined by using morphometrical method in three ways:
1) mean number of AgNORs per nucleus counted on a 100 random nuclei, 2)
mean number of AgNORs per nucleus counted on 200-300 nuclei in fields chosen
by "the chess fields" method, and 3) mean number of AgNORs on the nuclear
surface unit. A significant difference in mean number of AgNORs per nucleus
and on unit of the nuclear surface between estrogen receptor positive (ER+)
and estrogen receptor negative (ER-) invasive ductal breast carcinomas
was observed (p<0.001). The mean number of AgNORs was higher in ER–
than in ER+ invasive ductal breast carcinomas. Despite interobserver variability
in counting AgNORs, comparison was completed as well as the evaluation
of validity of three stated ways of quantification of nucleolar organizers.
Our results suggest more rapid proliferative activity in ER- than in ER+
breast cancers.
Key words: Ductal breast cancer,
argyrophilic nucleolar organiser regions (AgNORs), estrogen receptor, morphometry
KVANTIFIKACIJA ARGIROFILNIH NUKLEOLARNIH
ORGANIZATORA
U ESTROGEN RECEPTOR POZITIVNIM I ESTROGEN
RECEPTOR NEGATIVNIM DUKTALNIM KARCINOMIMA DOJKE
Kratak sadržaj: U ovom istraživanju analizirani su argirofilni nukleolarni
organizatori (AgNOR) kod 20 estrogen receptor pozitivnih (ER+) i 16 estrogen
receptor negativnih (ER-) invazivnih duktalnih karcinoma dojke. Kvantifikacija
AgNOR-a je sprovedena morfometrijskom metodom na tri načina: 1) određivanjem
prosečnog broja AgNOR-a po jedru ćelije brojanjem na 100 slučajno odabranih
jedara, 2) određivanjem prosečnog broja AgNOR-a po jedru ćelije brojanjem
na 200-300 jedara na svakom drugom vidnom polju i određivanjem prosečnog
broja AgNOR-a na jedinicu površine jedra. Nađena je statistički signifikantna
razlika u prosečnom broju AgNOR-a po jedru i po jedinici jedarne površine
između ER+ i estrogen receptor negativnih ER- invazivnih duktalnih karcinoma
dojke (p < 0,001). ER - karcinomi dojke imaju signifikantno veći broj
AgNOR-a po jedru u odnosu na ER + karcinome. Izvršena je komaparacija sva
tri načina kvantifikacije. Rezultati ovog istraživanja ukazuju na veću
proliferativnu aktivnost u ER- u odnosu na ER+ karcinome dojke.
Ključne reči: duktalni karcinom
dojke, argirofilni nukleolarni organizatori (AgNOR), estrogeni receptori,
morfometrija
