Vol.12, No 2, 2005 pp. 81 - 84
UC 612.41:611.71:616.155.194
ASTEREOLOGICAL EXAMINATION OF
IMMATURITY
OF
MEGALOBLAST CELL NUCLEI OF BONE MARROW IN
PERNICIOUS ANEMIA
Lana Mačukanović-Golubović1,
Gorana
Rančić2, Mladen Milenović1,
Dragan Mihailović3, Gordana Kostić4
1Clinic of Haematology,
2Institute of Histology and
Embryology
3Institute of Pathology
4Clinic of Pediatrics, Medical
Faculty,
Niš, Serbia and Montenegro
Summary. In pernicious anemia (PA) proliferation of hematopoetic
cells is disturbed due to impaired DNA synthesis. The bone marrow is
usually
hypercellular with dominant megaloblast transformation of erythrocyte
precursors.
Megaloblastic changes are present in all stadiums of erythrocyte
development.
The aim of our study was to apply astereological methods in order to
estimate
morphological immaturity of megaloblastic cell nuclei in the bone
marrow
in PA. Patients diagnosed with PA (58) and treated at the Clinic of
Hematology,
Niš, during the period 2000-2004 were divided into three groups
according
to the pathological finding of the gastric mucosa. Astereological
examination
of megaloblastic cells of the bone marrow was performed using a B 100
grid
system. The absolute nuclear surface, absolute contour nuclear density
and form factor of megaloblastic nuclei was determined. A significant
increase
in the absolute surface and contour density of nuclei of the examined
cells
was found in all groups, while the nuclear form factor showed a
statistically
significant reduction. It could be concluded that astereological
changes
in erythroid elements can be used for detection of cells with impaired
function.
Key words: Pernicious anemia, bone marrow, erythroid elements,
astereological analysis
ASTEREOLOŠKO ISPITIVANJE NEZRELOSTI
JEDARA
MEGALOBLASTA KOSTNE SRŽI U PERNICIOZNOJ ANEMIJI
Kratak sadržaj: Proliferacija hematopoetskih ćelija u
pernicioznoj
anemiji (PA) je poremećena zbog neadekvatne sinteze DNA. Kostna srž je
obično hipercelularna i dominira megaloblastna transformacija
eritrocitnih
prekursora. Megaloblastne promene su prisutne u svim stadijumima
eritrocitnog
razvića. Cilj rada je bio kvantifikovati morfološki vizuelizovanu
nezrelost
jedara megaloblastnih ćelija kostne srži u PA korišćenjem astereoloških
metoda. Obuhvaćeno je 58 pacijenata sa PA u periodu 2000-2004. godine
koji
su podeljeni u tri grupe prema patološkom nalazu želudačne sluzokože.
Astereološko
ispitivanje megaloblastnih ćelija kostne srži obavljeno je korišćenjem
mrežastog sistema B100. Određivani su apsolutna nuklearna površina,
apsolutna
konturna gustina i oblikovni faktor jedra megaloblasta. Naši rezultati
ukazuju da apsolutna površina i konturna gustina jedra ispitivanih
ćelija
rastu statistički značajno u svim grupama, dok oblikovni faktor jedra
pokazuje
statistički značajno smanjenje. Uzimajući u obzir astereološke promene
eritroidnih elemenata može se diskutovati i zaključiti da je funkcija
ovih
ćelija neadekvatna.
Ključne reči: Perniciozna anemija, kostna srž, eritroidni
elementi,
astereološke analize
