Vol.12, No 1, 2005 pp. 44 - 46
UC 615.33
UV/VIS ANALYSIS AND ANTIMICROBIAL
ACTIVITY
OF STREPTOMYCES ISOLATES
Slavica B. Ilić, Sandra S. Konstantinović, Zoran B. Todorović
Faculty of Technology, University
of Niš, Leskovac, Serbia and Montenegro
E-mail: ilicslavica@ yahoo.com
Summary. Twenty different Streptomyces isolates were gained from
the soils of Southeastern Serbia. Nine isolates showed a strong
activity
against Bortrytis cinerea, a parasite found in domestic vine. These
isolates
where extensively studied for their in vitro anti-microbial activity
against
Gram-positive and Gram-negative bacteria and yeasts. The results
indicated
that obtained isolates were highly active against Bortrytis cinerea,
Herpes
simplex and Candida albicans with an inhibition zone at >= 31mm.
Five of
these isolates were identified as Streptomyces hygroscopicus (SH100,
SH101,
SH102, SH103, SH104). The UV spectra of the culture extracts for the
active
isolates showed absorbance peaks ranging between 221 and 240 nm. Two
bioactive
regions were detected on the TLC plate (Rf 0.70 and 0.88). The UV
spectra
of the active compounds in methanol showed peaks at 217 and 221nm.
Key words: Anti-microbial activity, antibiotics, Streptomyces
UV/VIS ANALIZA ANTIMIKROBNA
AKTIVNOST
STREPTOMYCES IZOLATA
Kratak sadržaj: Dvadeset različitih izolata Streptomyces je
dobijeno
iz tla sa područja jugoistočne Srbije. Devet izolata pokazuje jaku
aktivnost
na Bortrytis cinerea, parazit nadjen u domaćem vinu. Ispitivana je
antimikrobna
aktivnost ovih izolata in vitro na Gram-pozitivne, Gram-negativne
bakterije
i gljive. Rezultati pokazuju da dobijeni izolati imaju veliku aktivnost
na Bortrytis cinerea, Herpes simplex i Candida albicans sa zonama
inhibicije >= 31 mm. Pet od ovih izolata su identifikovani kao
Streptomyces hygroscopicus
(SH100, SH101, SH102, SH103, SH104). UV spektri ekstrakta kulture za
aktivne
izolate pokazuju pik apsorbancije u oblasti izmedju 221 i 240 nm. Dva
bioaktivna
regiona su detektovana pomoću TLC (Rf 0,7 i 0,88). UV spektar
aktivnih
jedinjenja u metanolu pokazuje pikove na 217 i 221 nm.
Ključne reči: Antimikrobna aktivnost, antibiotici, Streptomyces
