Vol.11, No 3, 2004 pp. 143 - 147
UC 612.086
COLORIMETRIC DETERMINATION OF DIAZO-DYES
AFFINITY
FOR ELASTIC TISSUE AND MODEL PROTEINS
Mirjana Abramović, Goran Nikolić
Department of Chemistry, Faculty of Medicine, Niš, Serbia and Montenegro
Summary. A model system was established which could serve for the
quick and reliable estimation of the possibility to use aromatic dyes in
staining elastic fibers and lamellas. Specially prepared samples of aorta
wall tissue (taken from the corpse material of a healthy male, aged 31)
and model proteins (collagen and elastin) were stained with acid diazo-dyes
applied in water solution, 70% ethanol and dimethyl-sulfoxide. The amount
of bonded dye was determined quantitatively according to the difference
in absorbance of dye solution before and after the sample staining. The
results of colorimetric measurements clearly showed a good correlation
between the chemical structure of dyes and their binding to substrate,
thus indicating the possibility that this model system cuold be used for
simple and quick choice of dye solvent as well as particular aromatic
dyes which should be used for elastic fibers dyeing in light microscopy.
Key words: Elastic tissue, elastin, collagen, diazo-dyes, colorimetric
analysis
KOLORIMETRIJSKO ODREĐIVANJE AFINITETA
DIAZO-BOJA
ZA ELASTIČNO TKIVO I MODEL PROTEINE
Kratak sadržaj: Na osnovu istraživanja uspostavljen je model sistem
koji će poslužiti za brzu i pouzdanu procenu mogućnosti upotrebe aromatičnih
boja za bojenje elastičnih vlakana. Posebno pripremljeni uzorci tkiva aortnog
zida i model proteina (elastina i kolagena), bojeni su diazo-bojama koje
su rastvarane u vodi, 70%-etanolu odnosno dimetil-sulfoksidu. Količina
vezane boje određivana je kvantitativno, na osnovu razlike u absorbanciji
rastvora boje, pre i posle bojenja uzoraka. Rezultati sugerišu mogućnost
upotrbe ovakvog model sistema za jednostavan i brz izbor rastvarača za
boje, kao i diazo-boja koje bi se koristile u bojenju preseka u svetlosnoj
mikroskopiji.
Ključne reči: Elastično tkivo, elastin, kolagen, diazo-boje,
kolorimetrijska analiza
