Vol.11, No 2, 2004 pp. 63 - 68
UC 612.43:612.67
MORPHOLOGY AND HISTOCHEMICAL CHARACTERISTICS
HUMAN PINEAL GLAND ACERVULI DURING THE AGING
Svetlana Antić1, Ivan Jovanović1,
Natalija Stefanović1, Snežana Pavlović1,
Gorana Rančić2, Slađana Ugrenović1
1Department of Anatomy of Medical Faculty,
Niš, Serbia and Montenegro
2Department of Histology and Embryology
of Medical Faculty, Niš, Serbia and Montenegro
Summary. Pineal acervuli are calcificated structures, present in
gland's parenchyma as well as in its leptomeningeal capsula.. The aim of
our research was to determine the morphological and histochemical characteristics
of pineal acervuli during the aging process. During our research, 30 corpses
pineal glands were analyzed with light microscope. Their age ranged from
20 to 82, and they were classified into three age groups. Samples were
processed by standard histological method, and thereafter stained with
HE stain, PAS and AB PAS stain, modified aldehyde fuchsine stain, resorcin
fuchsin stain, Gordon Sweet method and Mallory's trichrome connetctive
tissue stain. Acervuli stained blue on HEstained sections. They were PAS
and AB PAS positive. On aldehyde fuchsin stained sections they were violet
and on resorcin fuchsine stained sections they were red colored. Acervuli
had blue periphery and red core on Mallory's trichrome connective tissue
stained sections. With Gordon Sweet's method they had yellow colored core
and black colored periphery. The first age group acervuli were small with
regular round or oval shape. They were localized at gland's periphery.
Their size increased during the aging, and their shape became more irregular.
Finally, large mulberry like acervuli were formed in the third age group
probably as result of the conglomeration process. Hence, pineal acervuli
are the structures which are consisted from inorganic and organic substances.
During the aging they become larger with more irregular shape, which finally
lead to mulberry like composite pineal acervuli formation which dominate
in the pineal parenchyma.
Key words: Pineal gland, acervuli, morphology, histochemistry
MORFOLOGIJA I HISTOHEMIJSKE KARAKTERISTIKE
ACERVULUSA PINALNE ŽLEZDE ČOVEKA TOKOM STARENJA
Kratak sadržaj: Acervulusi pinealne žlezde su kalcifikovane strukture,
koje se uočavaju u njenom parenhimu i leptomeningealnom omotaču. Cilj našeg
istraživanja bio je da se sagledaju morfološke i histohemijske karakteristike
acervulusa tokom starenja čoveka. Analiza tkiva pinealne žlezde 30 kadavera
starosti od 20 do 82 godine koji su klasifikovani u tri starosne grupe
izvršena je uz pomoć svetlosnog mikroskopa. Tkivo je obrađivano standardnim
histološkim metodom i zatim je bojeno HE, PAS, AB PAS, rezorcin fuksin,
modifikovanim aldehid fuksin, Gordon Sweet-ovim metodom i trihromnim bojenjem
za vezivno tkivo - modifikacija po Mallory-u. Na preparatima bojenim HE
acervulusi su se bojili plavo. Oni su PAS i AB PAS pozitivne strukture.
Aldehid fuksinom se boje ljubičasto, a rezorcin fuksinom crvenkasto. Primenom
Gordon Sweet-ove metode središte im se boji žuto, a periferija crno. Na
preparatima bojenim trihromnim bojenjem modifikacija po Mallory-u periferija
im se boji plavo a centar crveno. Acervulusi prve starosne grupe su sitni
pravilnog okruglog ili ovalnog oblika, lokalizovani na periferiji žlezde.
Tokom starenja, dolazi do porasta veličine acervulusa pri čemu njihov oblik
postaje nepravilniji što na kraju u trećoj starosnoj grupi rezultira formiranjem
krupnih kupinastih acervulusa, koji nastaju najverovatnije kao rezultat
procesa konglomeracije. Prema tome, acervulusi su strukture koje se sastoje
iz organskih i neorganskih materija. Tokom starenja oni postaju veći a
njihov oblik nepravilniji što, na kraju dovodi do stvaranja složenih kupinastih
struktura koje dominiraju parenhimom žlezde.
Ključne reči: Pinealna žlezda, acervulusi, morfologija, histohemija
