Vol. 10, No 2, 2003 pp. 84 - 87
UC 616-092.9:591.434
REGIONAL DIFFERENCES IN MOTOR RESPONSIVENES
TO HYOSCINE BUTYLBROMIDE
IN RABBIT ISOLATED SMALL AND LARGE INTESTINE
Mirjana Radenković, Ranka Samardžić, Slavimir Veljković, Suzana
Branković, Milkica Nešić, Dragana Veličković
Department of Physiology Medical faculty, Niš, Serbia
Summary. The effect of hyoscine butylbromide (scopolamine butylbromide),
a competitive nonselective antagonist of acetylcholine at muscarinic receptors,
on the isolated rabbit duodenum, ileum and colon was investigated. Hyoscine
butylbromide (9x10-9M - 910-6M)
decreased in a concentration-dependent manner the amplitude of the spontaneous
pendular movements of the rabbi-isolated duodenum (r=0.99; p<0.001),
ileum (r=1; p<0.001) and colon (r=0.98; p<0.001).
Hyoscine butylbromide decreased the tone of the spontaneous pendular
movements. The effect on the tone of the duodenum was not concentration-dependent.
It decreased the tone of the duodenum by 20.67% ą 5.99 (r=0.69; p>0.05);
(F=1.38; p>0.05) and had no significant effect on the ileal tone. However,
the tone of distal colon was decreased in a concentration-dependent manner
(r=0.98; p<0.001).The frequency of the pendular movements was the least
sensitive to hyoscine butylbromide. The frequency of duodenal and ileal
contraction did not change. However, the frequency of pendular movements
of the colon was slightly, but concentration-dependently (r=0.93; p<0.01)
decreased.
It is concluded that hyoscine butylbromide reduced the amplitudes of
pendular movements most effectively in all parts of the intestines.
The inhibitory effect on the tone was most pronounced on the colonic contractions,
whereas the duodenal tone was less sensitive, and the ileal tone was unaffected.
Only the frequency of the pendular movements of the rabbit
colon was sensitive to the muscarinic blockade. It is suggested that hyoscine
butylbromide predominantly acted on M1 muscarinic receptors.
Key words: Pendular movements, muscarinic receptors, hyoscine
butylbromide, rabbit, duodenum, ileum, colon
REGIONALNE RAZLIKE U MOTORNOJ AKTIVNOSTI
IZOLOVANIH SEGMENATA TANKOG I DEBELOG CREVA KUNIĆA IZAZVANE HIOSCIN BUTILBROMIDOM
Kratak sadržaj: Ispitivan je uticaj buskopana (hioscin butilbromida),
neselektivnog blokatora muskarinskih receptora, na motornu aktivnost izolovanih
segmenata duodenuma, ileuma i kolona kunića.
Hioscin butilbromid (scopolamin butilbromid, buskopan) (9x10-9M
- 9x10-6M) koncentracijski zavisno smanjuje
amplitudu pendularnih pokreta izolovanog duodenuma (r=0,99; p<0,001),
ileuma (r=1; p<0,001) i kolona (r=0,98; p<0,001) kunića. Hioscin
butilbromid ne deluje podjednako na tonus tankog i debelog creva. On smanjuje
tonus duodenuma prosečno za 20,67% ą 5,99 (r=0,.69; p>0,05); (F=1,38; p>0,05).
Efekat nije koncentracijski zavisan. U istim koncentracijama nema uticaja
na tonus ileuma, a koncentracijski zavisno snižava tonus distalnog kolona
kunića (r=0,98; p<0,001). Najslabiji efekat ispoljava na frekvenciju
pendularnih pokreta. Ne menja frekvenciju duodenuma i ileuma, a neznatno,
ali koncentracijski zavisno, smanjuje frekvenciju pendularnih pokreta kolona
(r=0,93; p<0,01).
Možemo zaključiti da hioscin butilbromid najsnažnije redukuje amplitudu
pendularnih pokreta celog digestivnog kanala. Slabije deluje na tonus gornjih
partija creva, a snažno smanjuje tonus kolona. Najslabiji uticaj ispoljava
na frekvenciju pendularnih pokreta. Na osnovu naših nalaza možemo pretpostaviti
da efekte ostvaruje pretežno preko muskarinskih M1 receptora.
Ključne reči: Pendularni pokreti, muscarinski receptori, hioscin
butilbromid, kunić, duodenum, ileum, kolon
