Vol. 10, No 2, 2003 pp. 76 - 78
UC 616.34-006[616-071:577.15
DIAGNOSTIC IMPORTANCE OF ADENOSINE DEAMINASE
ACTIVITY
FOR PROGRESSION AND INVASION OF HUMAN COLON
TUMORS
G. Kocić1, G. Stanojević2,
A. Nagorni3, B. Branković2,
D. Pavlović1, T. Jevtović1
1Institute of Biochemistry, Faculty
of Medicine Niš, Serbia and Montenegro
2Clinic for Surgery, Faculty of Medicine
Niš, Serbia and Montenegro
3Clinic for Gastroentherology, Faculty
of Medicine Niš, Serbia and Montenegro
Summary. The aim of the study was to explore the usefulness of adenosine
deaminase (ADA) activity as a marker of colon tumor progression and to
find a quantitative expression of ADA in mucosa of colon carcinoma, polyps,
adjacent to tumors, or mucosa from anastomosis after surgical resection.
The highest ADA activity was obtained from mucosa adjacent to carcinoma
compared to healthy tissue. The ADA activity from polyp tissue increased
slightly, compared to the activity of further healthy tissue, the enzyme
activity from anastomosis was also higher than the activity of corresponding
healthy tissue. The highest enzyme activity of mucosa adjacent to carcinoma
suggest that ADA may be included in tumor growth and progression and may
be of importance as an early marker of colon carcinoma invasion. Obtained
data could be useful in assessing the margins for surgical resection of
colorectal carcinoma and assessing prognosis and clinical outcome of the
disease after surgical resection, together with known clinicopathologic
parameters.
Key words: Adenosine deaminase, colon carcinoma
DIJAGNOSTIČKI ZNAČAJ AKTIVNOSTI ADENOZIN
DEZAMINAZE
ZA PROGRESIJU I INVAZIJU HUMANOG TUMORA KOLONA
Kratak sadržaj: Cilj studije je bio da se ustanovi značaj praćenja
aktivnosti adenozin dezaminaze (ADA) kao markera progresije tumora kolona
i odredi kvantitativna ekspresija ADA u karcinomu kolona, polipu, tkivu
neposredno uz tumor ili u anastomozi nakon hiruške resekcije tumora. Najveća
aktivnost enzima je pokazana u tkivu neposredno uz tumor u poređenju sa
zdravim tkivom. Povećanje aktivnosti ADA je bilo slabije izraženo u tkivu
polipa a porast je bio i u anastomozi u poređenju sa kontrolnim zdravim
delovima. Najizraženija aktivnost enzima u tkivu neposredno uz karcinom
ukazuje da bi ADA mogla da bude uključena u rast i progresiju tumora i
da bi mogla biti od koristi kao rani marker invazije karcinoma kolona.
Dobijeni rezultati bi mogli da budu od koristi pri utvrđivanju granica
hiruške resekcije kolorektalnog karcinoma, u prognozi i kliničkom ishodu
oboljenja nakon hiruške resekcije, zajedno sa poznatim klinikopatološkim
nalazima.
Ključne reči: Adenozin dezaminaza, karcinom kolona
