Vol. 10, No 2, 2003 pp. 62 - 68
UC 616-036.21:616.61(497):[612.461.1+616.15
ANALYSIS OF OCHRATOXIN A IN SERUM AND
URINE OF INHABITANTS FROM AN AREA WITH BALKAN ENDEMIC NEPHROPATHY:
A ONE MONTH FOLLOW UP STUDY
T. Petkova-Bocharova 1, M. Castegnaro
2a,
A. Pfohl-Leszkowicz 3*, L. Garren 2,
F. Grosso 4, I. Nikolov 1,
T. Vrabcheva 5, S. Dragacci 4,
I.N. Chernozemsky 1
1 Institute of Oncology, - 6 Plovdivsko
Pole, Sofia Darvenitza, Bulgaria
2 Unit of Gene-Environment Interactions,
International Agency for Research on Cancer, 150 cours Albert Thomas,
69372 Lyon Cedex 08, France, aConsultant, Les Collanges, 07240
Saint Jean Chambre, France
3 Laboratoire de Toxicologie et Sécurité
Alimentaire, Ecole Nationale Supérieure d'Agronomie,
Avenue de l'Agrobiopole, BP 107, F-31326 Auzeville-Tolosane,
Toulouse, France
* corresponding author: leszkowicz@ensat.fr
4 Unité des Toxines Microbiennes, Agence
Française de Sécurité Sanitaire des Aliments, Maison Alfort, France
5 National Center of Hygiene, Mycotoxicological
Laboratory, 15 Dimitar Nestorov Street,1431 Sofia, Bulgaria
Summary. In the 1950s a series of publications from Bulgaria, Yugoslavia
and Romania described a kidney disease occurring in geographically limited
areas of these three Balkan countries. Several studies implicated a mycotoxin
etiology, and particularly ochratoxin A. In Bulgaria, the exposure of populations
to OTA was supported by a very high prevalence of OTA levels exceeding
2 ng/ml in the blood of affected population. OTA has also been found more
often in the urine of people living in BEN-endemic villages than in those
in non-endemic villages, and the highest levels were seen in patients with
BEN or urinary tract tumors. In the present study we follow up the blood
and urine OTA levels of some population from the Bulgarian high incidence
area of BEN, not affected by any renal diseases. Samples were thus collected
for one month, at the beginning of week one and then at the end of each
week. This study strengthens our previous results and demonstrate that
the population in the Balkans is more exposed to OTA than the general population
in Europe.
Key words: Balkan Endemic Nephropathy, ochratoxin A, serum &
urine analysis
ANALIZA OCHRATOXIN-a U SERUMU I URINU
STANOVNIŠTVA IZ OBLASTI
SA BALKANSKOM ENDEMSKOM NEFROPATIJOM: JEDNOMESEČNA
STUDIJA
Kratak sadržaj: U toku 1950-ih serija publikacija iz Bugarske, Jugoslavije
i Rumunije opisala je bolest bubrega koja se javlja u geografski definisanim
regijama ove tri balkanske zemlje. Nekoliko studija upućuje na etiološku
povezanost sa mycotoxin-om i delimično sa ochratoxin-om A. Izloženost populacije
OTA u Bugarskoj je praćena visokom prevalencijom nivoa OTA koji prevazilazi
2 ng/ml u krvi pogođene populacije. OTA je takođe nađen češće u urinu ljudi
koji žive u BEN-endemskim u poređenju sa neendemskim naseljima, a najviše
vrednosti se beleže kod obolelih od BEN ili tumora urotrakta. U ovoj studiji
mi pratimo nivo OTA u krvi i urinu populacije sa visokom incidencom BEN
u Bugarskoj bez znakova bolesti bubrega. Uzorci su sakupljani u toku jednog
meseca početkom i krajem nedelje. Ispitivanje je potvrdilo naše prethodne
rezultate koji pokazuju da je populacija na Balkanu izloženija delovanju
OTA no što je to slučaj sa populacijom u Evropi.
Ključne reči: Balkanska endemska nefropatija, ochratoxin A,
analize urina i seruma
