Vol. 10, No 1, 2003 pp. 22 - 26
UC 616.275:547.72
IMMUNOCYTOCHEMICAL AND ULTRASTRUCTURAL
CHARACTERISTICS OF PANCREATIC B-CELLS IN RATS TREATED WITH FURFURAL
Snežana Cekić1, Miloš Filipović2,
Milkica Nešić1 , Suzana Branković1,
Milan Ćirić1, Katarina Katić3,
Svetlana Kamenov4, Oliver Dimitrijević
1Institute of Physiology, Faculty of
Medicine, University of Niš, 2Center for
Pulmonary Diseases, Niš
3Institute of Pathology, Faculty of
Medicine, University of Niš, 4Health Center
of Niš, Serbia
Summary. Furfural (C5H4O2),
unsaturated cyclic aldehyde, is a well-known hepatotoxic substance that
rapidly oxidizes into pyromucic acid (C4H3OCOOH)
that is responsible for damage to liver parenchyma and development of hepatic
insufficiency. In animals treated with this aldehyde, severe degenerative
and necrotic alterations may occur, leading to cirrhosis development and
hepatic carcinoma. Considering numerous anabolic, catabolic, detoxifying,
and excretory functions of the liver, as well as a functional relation
between the liver and the pancreas, we set out to examine the effect of
furfural upon endocrine B-cells of the insula. An analysis was done of
immunocytochemical and ultrastructural properties of pancreatic B-cells
in rats treated with furfural for two months. In the treated animals, hypogranulation
of the examined cells was found along with their nesidioblastosis in the
exocrine pancreas. A conclusion can be drawn that furfural reduces hormone
deposit in B-cells. Ultrastructural analysis of these cells shows that
they contain granules of wide-ranging maturity, density, size, and shape,
whilst the smooth endoplasmic reticulum is edematous and dilates tubules.
Key words: Furfural, insular B-cells, insulin
IMUNOCITOHEMIJSKE I ULTRASTRUKTURNE KARAKTERISTIKE
B ĆELIJA PANKREASA U PACOVA TRETIRANIH FURFURALOM
Kratak sadržaj: Furfural (C5H4O2),
nezasićeni ciklični aldehid, je dobro poznata hepatotoksična supstanca
koja se u organizmu brzo oksidiše u piromucinsku kiselinu (C4H3OCOOH)
a ona je odgovorna za oštećenje parenhima jetre i razvoj hepatične insuficijencije.
U životinja tretiranih ovim aldehidom nastaju teške degenerativno-nekrotične
promene hepatocita koje dovode do razvoja ciroze i hepatičnog karcinoma.
Obzirom na brojne uloge jetre (anaboličke, kataboličke, u detoksikaciji
i ekskreciji), kao i funkcijski odnos između jetre i pankreasa, ispitivali
smo ulogu furfurala na beta ćelije pankreasa. Ispitivana su imunohistohemijska
i ultrastrukturalna svojstva beta ćelija pankreasa pacova tretiranih dva
meseca furfuralom. Kod netretiranih pacova nađeni su hipogranulacija beta
ćelija i porast broja ćelija egzokrinog pankreasa. Može se zaključiti da
furfural redukuje depoe hormona u B ćelijama. Ultrastrukturna analiza ovih
ćelija pokazuje da one sadrže granule različite zrelosti, gustine, veličine
i oblika dok je glatki endoplazmatski retikulum edematozan sa dilatiranim
tubulima.
Ključne reči: Furfural, B ćelije insula, insulin
