Vol. 9, No 2, 2002 pp. 171 - 174
UC 591.438+591.81 
CHARACTERISTICS OF LAMELLAR BODIES IN PERITONEUM
Biljana B. Stojimirović1, Miljana M. Obradović3, Dušan P. Trpinac3,
Drago D. Milutinović2, Dragan I. Obradović4, Vidosava B. Nešić1
1Institute of Nephrology and 2Urology , Clinical Center of Serbia, Belgrade
3Institute of Histology, 4Institute of Pharmacology, Toxicology and Clinical Pharmacology,
School of Medicine, University of Belgrade, Yugoslavia
Summary. We examined 20 healthy persons (kidney donors). Small samples of parietal peritoneum were taken during the surgical operation for kidney extirpation. To avoid artificial damage owing to the extreme fragile of peritoneal tissue, the biopsies were treated with special care. The samples were prepared in the standard way for study by transmission electron microscopy. In order to preserve the fine structure of lamellar bodies, we used a fixative mixture including gluthar aldehyde and tannic acid.
The aim of our study was to investigate the localization and ultrastructure of lamellar bodies of peritoneum in order to contribute to finding out their physiological function and importance for this tissue.
Special round or oval multilaminar structures – lamellar bodies could be recognized among microvilli of peritoneal mesothelial cells apical plasma membrane. Lamellae were organized as concentric bilaminar structures – one of the layers was osmiophilic (dark, electron-dense), the next one was electron-lucent. The lamellar bodies were noticed in mesothelial cells cytoplasm, and were released from the cells by process of exocytosis. For some time after exocytosis, lamellar bodies with regular structure were recognized among the microvilli. Afterwards, probably because of the process of swelling, lamellar bodies had been loosing the regular geometrical structure and were recognized on our photomicrographs with only a few lamellae and a free central part,. Lamellar bodies could also be seen between two adjacent mesothelial cells, above and in the cellular junctions, possibly contributing to the intercellular sealing and prevention of paracellular transport. We recognized lamellar bodies in lamina propria just below the mesothelial cells, as well as in endothelial cells of peritoneal blood vessels.
Key words:  Mesothelium, endothelium, peritoneum, ultrastructure, lamellar bodies
KARAKTERISTIKE LAMELARNIH TELA U PERITONEUMU
Kratak sadržaj: Biopsije peritoneuma uzete su od 20 zdravih dobrovoljaca, donora bubrega za transplantaciju. Isečci tkiva dobijani su tokom hirurškog zahvata uzimanja bubrega. Uzorci su standardno pripremljeni za transmisionu elektronsku mikroskopiju (TEM). Da bi se sačuvala struktura lamelarnih tela, koristili smo mešavinu fiksativa glutar aldehida i taninske kiseline.
Cilj ovog istraživanja bio je da se detaljno ispita morfologija i lokalizacija lamelarnih tela peritoneuma zdravih osoba da bi se doprinelo utvrdjivanju njihove uloge.
Multilaminarne strukture, lamelarna tela, okruglog ili ovalnog oblika, zapažala su se izmedju mikrovila apikalne membrane mezotelnih ćelija peritoneuma. Lamele su bile organizovane kao koncentrične bilaminarne strukture – jedna lamela bila je tamna (veće elektronske gustine), dok je susedna bila svetla (male elektronske gustine). Lamelarna tela su nadjena i u citoplazmi mezotelnih ćelija, iz kojih su sekretovana egzocitozom. Neko vreme nakon egzocitoze, izmedju mikrovila su zapažena lamelarna tela regularne strukture, a zatim, verovatno kao posledica procesa bubrenja, lamelarna tela gube pravilnu geometrijsku strukturu i mi ih, na fotomikrografijama, uočavamo sa samo nekoliko lamela na periferiji i praznim centralnim delom. Lamelarna tela mogu takodje biti zapažena izmedju dve susedne mezotelne ćelije, iznad ili u okviru medjućelijske veze, sa mogućom ulogom sprečavanja paracelularnog transporta. Lamelarna tela takodje su nadjena u vezivnom tkivu kao i u endotelnim ćelijama krvnih sudova lamine proprije peritoneuma.
Ključne reči: Mezotel, endotel, peritoneum, ultrastruktura, lamelarna tela