Vol. 8, No 1, 2001 pp. 54 - 58
UC  616.23 
IRON CONTENT IN SERUM AND PULMONARY TISSUE OF RABBITS
WITH EXPERIMENTALLY INDUCED PULMONARY EMPHYSEMA
Snežana Žunić1, Danica Đarmati2, Svetlana Žunić3, Gordana Đorđević-Denić1
1Department of Pathophysiology, Medical Faculty, Belgrade, Yugoslavia
2Health Protection Institute of Serbia, Belgrade, Yugoslavia
3Institute for Nuclear Medicine, Oncology Unit, Clinical Center of Serbia, Belgrade, Yugoslavia
E-mail: nzunic@ptt.yu
Summary. The pathogenesis of pulmonary emphysema is uncompletelly understood. As a catalyzer of some oxidants production iron is involved in oxidant/antioxidant balance disturbances, associated with oxidative stress injury of pulmonary tissue. That's why the iron content was studied in serum and pulmonary tissue of rabbits with pulmonary emphysema induced by hipercholesterolemic diet. Experimental animals were divided into three groups (of 10 animals each): hypercholesterolemic (H)-on hypercholesterolemic diet (oil solution of crystalline cholesterol), oil (O)-on oil diet, and control (C)-on standard diet for that animal species. Pulmonary emphysema was pathohistologically confirmed. Iron content in serum and pulmonary tissue was determined using atomic absorptive spectrophotometric method. There is a significant increase in serum iron content in H in comparison with C group of rabbits. Pulmonary tissue iron content is found to be significantly decreased in H and O compared to C group of investigated animals.
The changes of iron content in serum and pulmonary tissue, in relation with pathohistological findings of pulmonary tissue, could be of certain importance in developing mechanisms of pulmonary emphysema induced by hypercholesterolemic diet.
Key words: Pulmonary emphysema, iron, hypercholesterolemic diet
SADRŽAJ GVOŽĐA U SERUMU I TKIVU PLUĆA KUNIĆA
SA EKSPERIMENTALNO INDUKOVANIM EMFIZEMOM PLUĆA
Kratak sadržaj: Patogeneza emfizema pluća je nepotpuno izučena. Kao katalizator procesa produkcije nekih oksidanasa gvožđe je uključeno u poremećaje ravnoteže oksidanasa/antioksidanasa, koji su povezani sa oksidativnim oštećenjem tkiva pluća. Stoga je određivan sadržaj gvožđa u serumu i tkivu pluća kunića sa emfizemom pluća indukovanim hiperholesterolskom dijetom.  Eksperimentalne životinje bile su podeljene u tri grupe (od po 10 životinja svaka): hiperholesterolsku (H)-na hiperholesterolskoj dijeti (uljani rastvor kristalnog holesterola), uljanu (U)-na dijeti uljem i kontrolnu (K)- na standardnoj dijeti za ovu životinjsku vrstu. Emfizem pluća je patohistološki potvrđen. Sadržaj gvožđa u serumu i tkivu pluća određivan je korišćenjem metode atomske apsorpcione spektrofotometrije. Registrovan je značajan porast sadržaja gvožđa u serumu H u poređenju sa K grupom kunića. Sadržaj gvožđa u tkivu pluća značajno je snižen u H i U u poređenju sa K grupom ispitivanih životinja.
Izmene u sadržaju gvožđa u serumu i tkivu pluća, povezane sa patohistološkim nalazima tkiva pluća, mogu biti od izvesnog značaja u mehanizmima razvoja plućnog emfizema uzrokovanog hiperholesterolskom dijetom.
Ključne reči: Plućni emfizem, gvožđe, hiperholesterolska dijeta