Vol. 8, No 1, 2001 pp. 54 - 58
UC 616.23
IRON CONTENT IN SERUM AND PULMONARY TISSUE
OF RABBITS
WITH EXPERIMENTALLY INDUCED PULMONARY EMPHYSEMA
Snežana Žunić1, Danica Đarmati2, Svetlana
Žunić3, Gordana Đorđević-Denić1
1Department of Pathophysiology, Medical Faculty, Belgrade,
Yugoslavia
2Health Protection Institute of Serbia, Belgrade, Yugoslavia
3Institute for Nuclear Medicine, Oncology Unit, Clinical
Center of Serbia, Belgrade, Yugoslavia
E-mail: nzunic@ptt.yu
Summary. The pathogenesis of pulmonary emphysema is uncompletelly
understood. As a catalyzer of some oxidants production iron is involved
in oxidant/antioxidant balance disturbances, associated with oxidative
stress injury of pulmonary tissue. That's why the iron content was studied
in serum and pulmonary tissue of rabbits with pulmonary emphysema induced
by hipercholesterolemic diet. Experimental animals were divided into three
groups (of 10 animals each): hypercholesterolemic (H)-on hypercholesterolemic
diet (oil solution of crystalline cholesterol), oil (O)-on oil diet, and
control (C)-on standard diet for that animal species. Pulmonary emphysema
was pathohistologically confirmed. Iron content in serum and pulmonary
tissue was determined using atomic absorptive spectrophotometric method.
There is a significant increase in serum iron content in H in comparison
with C group of rabbits. Pulmonary tissue iron content is found to be significantly
decreased in H and O compared to C group of investigated animals.
The changes of iron content in serum and pulmonary tissue, in relation
with pathohistological findings of pulmonary tissue, could be of certain
importance in developing mechanisms of pulmonary emphysema induced by hypercholesterolemic
diet.
Key words: Pulmonary emphysema, iron, hypercholesterolemic diet
SADRŽAJ GVOŽĐA U SERUMU I TKIVU PLUĆA
KUNIĆA
SA EKSPERIMENTALNO INDUKOVANIM EMFIZEMOM PLUĆA
Kratak sadržaj: Patogeneza emfizema pluća je nepotpuno izučena.
Kao katalizator procesa produkcije nekih oksidanasa gvožđe je uključeno
u poremećaje ravnoteže oksidanasa/antioksidanasa, koji su povezani sa oksidativnim
oštećenjem tkiva pluća. Stoga je određivan sadržaj gvožđa u serumu i tkivu
pluća kunića sa emfizemom pluća indukovanim hiperholesterolskom dijetom.
Eksperimentalne životinje bile su podeljene u tri grupe (od po 10 životinja
svaka): hiperholesterolsku (H)-na hiperholesterolskoj dijeti (uljani rastvor
kristalnog holesterola), uljanu (U)-na dijeti uljem i kontrolnu (K)- na
standardnoj dijeti za ovu životinjsku vrstu. Emfizem pluća je patohistološki
potvrđen. Sadržaj gvožđa u serumu i tkivu pluća određivan je korišćenjem
metode atomske apsorpcione spektrofotometrije. Registrovan je značajan
porast sadržaja gvožđa u serumu H u poređenju sa K grupom kunića. Sadržaj
gvožđa u tkivu pluća značajno je snižen u H i U u poređenju sa K grupom
ispitivanih životinja.
Izmene u sadržaju gvožđa u serumu i tkivu pluća, povezane sa patohistološkim
nalazima tkiva pluća, mogu biti od izvesnog značaja u mehanizmima razvoja
plućnog emfizema uzrokovanog hiperholesterolskom dijetom.
Ključne reči: Plućni emfizem, gvožđe, hiperholesterolska dijeta