Vol. 8, No 1, 2001 pp. 46 - 49
UC 618.81
THE EFFECT OF ETHANOL TREATMENT
ON SURFACE ALKALINE PHOSPHODIESTERASE I ACTIVITY
OF CULTURED HUMAN MESANGIAL CELLS
Vladisav Stefanović, Predrag Vlahović, Marina Mitić-Zlatković
Institute of Nephrology and Hemodialysis, Faculty of Medicine, Niš,
Yugoslavia
Summary. Human mesangial cells grown in culture have been employed
to investigate the interaction between expo-sure to ethanol and alkaline
phosphodiestase I activity at the plasma membrane of intact cells. Chronic
exposure of human mesangial cells to ethanol induced a dose-dependent rise
in enzyme activity. Similar effect was observed after the treatment of
mesangial cells with methanol. Short-term (1-3 h) exposure to ethanol was
also found to cause a dose-dependent stimulation in enzyme activity, but
an inhibition at concentrations higher than 0.5%. A possible mechanism
involving a change in membrane fluidity was proposed for the observed effects
of ethanol on mesangial cell surface alkaline phosphodiesterase I.
A direct role for ethanol in the pathogenesis of IgA nephropathy was
previously suggested, and his study opens a way to understand mesangial
cell membrane changes upon ethanol exposure.
Key words: Ethanol, mesangial cell, alkaline phosphodiesterase
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UTICAJ TRETIRANJA ETANOLOM KULTIVISANIH
HUMANIH MEZANGIJSKIH ĆELIJA NA AKTIVNOST EKTO-ALKALINE FOSFODIESTERAZE
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Kratak sadržaj: Humane mezangijske ćelije u kluturi su korišćene
za ispitivanje uticaja etanola na aktivnost alkalne fosfodiesteraze I u
ćelijskoj membrani. Hronično izlaganje mezangijskih ćelija etanolu dovodilo
je do dozno-zavisnog porasta enzimske aktivnosti. Sličan efekat dobijen
je izlaganjem mezangijskih ćelija metanolu. Kratkotrajno izlaganje (1-3
h) etanolu takođe je dovodilo do stimulacije enimske aktivnosti zavisne
od doze, međutim, u dozama većim od 0,5% nađena je inhibicija enzimske
aktivnosti. Za objašnjenje uticaja etanola na alkalnu fosfodiesterazu I
mezangijskih ćelija, kao mogući mehanizam delovanja predložena je promena
u fluidnosti plazma membrane.
Prethodne studije sugerisale su ulogu etanola u nastanku sekundarne
IgA nefropatije, a ovo ispitivanje otvara mo-gućnost za razumevanje pomena
u plazma membrani mezangijskih ćelija pod dejstvom etanola.
Ključne reči: Etanol, mezangijske ćelije, alkalna fosfodiesteraza
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