Vol. 8, No 1, 2001 pp. 1 - 5
UC 612.17
OXIDATIVE MODIFICATIONS OF PLASMA PROTEINS
IN DIFFERENT STAGES OF CHRONIC RENAL FAILURE
Jasmina Mimić-Oka, Tatjana Simić, Marija Plješa, Nadja Stupar, Sejdefa
Turković
Institute of Biochemistry, Faculty of Medicine, Belgrade, Yugoslavia
E-mail: okasn@ptt.yu
Summary. In this study we investigated oxidative modifications of
plasma proteins in non-dialyzed patients with varying degrees of chronic
renal failure (CRF), and in patients on regular haemodialysis treatment.
The results pre-sented here show that level of 2,4 dinitrophenylhydrazone-reactive
carbonyl derivatives (CRD), as a marker of oxida-tive modification of proteins,
increased in plasma of all chronic renal failure patients compared to healthy
subjects. Statistically significant positive correlation between the serum
creatinine level and plasma CRD content in CRF was observed. The concentration
of protein sulphydryl groups (P-SH) in plasma, which are important chain
breaking "sacrificial" antioxidant, were markedly reduced in individuals
with CRF compared to healthy subjects. However, the degree of renal impairment
does not appear to influence plasma P-SH in our data. The results presented
support the statement that in chronic renal failure accumulation of free
radical species and an unscheduled oxidation of sus-ceptible protein injury
occurs. Thus, chronic renal failure appears to a state of "carbonyl stress"
with potentially damaging proteins. It was suggested that carbonyl overload
in CRF might contribute to the development of long-term uraemic complications,
such as atherosclerosis, aging and amyloidosis.
Key words: Chronic renal failure, reactive carbonyl derivatives,
thiol groups, oxidative stress, free radicals
OKSIDATIVNE MODIFIKACIJE PROTEINA PLAZME
U RAZLIČITIM STADIJUMIMA HRONIČNOG OŠTEĆENJA BUBREGA
Kratak sadržaj: U radu je ispitivano oksidativno oštećenje proteina
krvne plazme u nedijaliziranih bolesnika sa različitim stepenom hroničnog
oštećenja bubrega i bolesnika koji se leče ponavljanim hemodijalizama.
Prikazani rezultati pokazuju da je u plazmi svih bolesnika sa hroničnom
insuficijencijom bubrega, u odnosu na zdrave osobe, značajno povećana
koncentracija 2,4 dinitrofenilhidrazon-reaktivnih karbonilnih derivata
(RCD), koji su jedan od markera oksidativne modifikacije proteina. Postoji
statistički značajna korelacija izmedju koncentracije kreatinina u serumu
i sadržaja RCD u plazmi hroničnih bubrežnih bolesnika. Sadržaj proteinskih
tiol grupa (P-SH), koje su značajni "žrtveni" antioksidanti u prekidanju
lanca reakcija izazvanih slobodnim radikalima, značajno je smanjena u plazmi
ispitivanih bolesnika. Medjutim, ne postoji povezanost izmedju stepena
oštećenja bubrega i smanjenja nivoa P-SH grupa. Prikazani rezultati govore
u prilog tvrdnje da se u hroničnom oštećenju bubrega akumuliraju slobodni
radikali koji mogu oksidativno modifikovati i oštetiti molekule proteina.
Oksidativno oštećenje proteina krvne plazme zabeleženo je već u ranim stadijumima
hroničnog oštećenja bubrega.
Ključne reči: Hronično oštećenje bubrega, karbonilne grupe,
tiol grupe, oksidativni stres, slobodni radikali
